Agilent Technologies 1100 Series Manual de Serviço descarregar pdf (Página 42)

42 1100 Series DAD and MWD Reference Manual

2 How to optmize the Detector

Optimizing Spectral Acquisition (DAD only)

Storage of all spectra consumes a lot of disk space. It is very useful to have all

spectra available during optimization of a method or when analyzing unique

samples. However when running many samples of the same type, the large size

of data files with all spectra may become a burden. The detector provides

functions to reduce the amount of data, yet retaining the relevant spectral

information.

Options for storing spectra

Range

Only the wavelength range where the compounds in your sample absorb

contains information that is useful for purity checks and library searches.

Reducing the spectrum storage range saves disk space.

Step

Most substances have broad absorbance bands. Display of spectra, peak purity

and library search works best if a spectrum contains 5 to 10 data points per

width of the absorbance bands. For anisic acid (the example used before) a

step of 4 nm would be sufficient. However a step of 2 nm gives a better display

of the spectrum.

None Store signals only. No peak purity checks nor library search

features can be used for samples analyzed with this setting.

Apex Only one spectrum per peak is stored; a peak must be higher than

the value entered in Threshold (see below).

Apex + baseline Stores spectra for peaks > threshold at the top and at baseline

before and after each peak recommended minimum to do library

searches with baseline corrected spectra.

Apex + slope +baseline Add spectra at the up and down slope; required to do a minimum

peak purity check.

All in peak All spectra during all peak > Threshold are stored; required for a

full peak purity check.

Every 2nd spectrum Only every second spectrum is stored, occupying only half the disk

space.

All All spectra are stored.

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Agilent Technologies 1100 Series Manual de Serviço Página 42