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34 1100 Series DAD and MWD Reference Manual

2 How to optmize the Detector

Traditionally LC analysis with UV detectors is based on comparing

measurements with internal or external standards. To check photometric

accuracy of the Agilent 1100 DAD/MWD it is necessary to have more precise

information on path lengths of the flow cells.

The correct response is:

expected response * correction factor

Please find below the details of the Agilent 1100 DAD/MWD flow cells:

Figure 8 Influence of Cell Path Length on Signal Height

Absorbance

Time (min)

Analysis of pesticide standard

10-mm optical path length

6-mm optical path length

Table 5 Correction factors for Agilent 1100 DAD/MWD flow cells

Flow cell type Cell volume Part number Path length

(nominal)

Path length

(actual)

Correction

factor

Standard flow cell 13 µl G1315-60012 10 mm 9.80 ± 0.07 mm 10/9.8

Semi-micro flow cell 5 µl G1315-60011 6 mm 5.80 ± 0.07 mm 6/5.8

80 Nanoliter flow cell kit 0.08 µl G1315-68716 6 mm 6.00 ± 0.02 mm 6/6

500 Nanoliter flow cell kit 0.5 µl G1315-68724 10 mm 10.00 ± 0.02 mm 10/10

High Pressure flow cell 1.7 µl G1315-60015 6 mm 5.75 ± 0.07 mm 6/5.75

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Agilent Technologies 1100 Series Manual de Serviço Página 34