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1100 Series Capillary LC System Manual 43

Optimizing Performance 2

The Fast Composition Change/Reconditioning Function

Purpose

The capillary pump and the micro well-plate sampler are recommended for

capillary LC applications. Capillary LC methods have very low column flow

rates, typically in the range of 1-20 µl/min. At such low flow rates,

re-equilibrating the system to the initial mobile phase composition between

automated gradient runs may require a long time. To conveniently

re-equilibrate the system between automated gradient runs, the Fast

Composition Change/Reconditioning function is implemented.

The Fast Composition Change/Reconditioning function is available only in a

system that includes both a capillary pump and a micro well-plate sampler.

This function can be set up to occur automatically between runs, and/or to

occur automatically after any manual composition change.

How the Function Works

Regardless of when it occurs, the Fast Composition Change/ Reconditioning

function is always a 2-step process:

1 The micro well-plate sampler needle is placed over the waste position of the

flushport. The pump delivers a high flow rate at the initial composition

defined in the current method. This flow is maintained for the Fast System

Flush time defined in the user interface. During this time, the system is

being re-equilibrated, up to the sampler needle outlet.

NOT E:

NOTE

The Fast Composition Change/Reconditioning function is available only when the

capillary pump is operated in the micro mode.

NOTE

The high flow rate used for Fast System Flush is not user-defined. For the Fast System

Flush, the pump automatically sets a predetermined maximum pressure limit. This pressure

limit is determined by the hardware configuration of the pump.

The flow rate used for Fast System Flush is the highest flow which can be delivered

without exceeding the pressure limit.

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Agilent Technologies 1100 Series Manual de Instruções Página 53