Agilent Technologies 1100 Series Manual de Instruções descarregar pdf (Página 42)

32 1100 Series Capillary LC System Manual

2 Optimizing Performance

Sampler issues

• For fast gradient use valve to bypass function after the sample is transferred

to the column. This function results in smaller delay times and sharper

gradient curves.

• When doing automated gradient runs, use the fast composition

change/reconditioning function to equilibrate the system between runs.

Column thermostat issues

• Use the column brackets to put the column in contact with the heat

exchanger.

• Do not use the solvent pre-heating path (heat exchanger in the column

compartment) when you are working with capillary columns. The

dispersion will be too high.

DAD issues

• At very low flow rates, bubbles might form in the cell due to low pressure in

the cell. This might cause the detector signal to have spikes and noise.

Adding a 50 µm capillary to the outlet of the cell can reduce this effect.

• To avoid cell damage due to overpressure, set the upper pressure limit to

50 bar greater than the typical operating pressure.

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Agilent Technologies 1100 Series Manual de Instruções Página 42