Agilent Technologies 1100 Series Manual de Instruções descarregar pdf (Página 35)

1100 Series Capillary LC System Manual 25

Installing your Capillary LC System 1

Conditioning the System Under Method Conditions

If you wish to condition the analytical column at this time, leave the column

installed in the TCC.

If you do not wish to condition the analytical column at this time, then remove

the column. In the TCC, connect the sampler-to-column capillary

(G1375-87304) directly to the DAD flow cell inlet capillary (G1315-68703).

This connection can be made with a ZDV fitting (0100-0900)

Enter your method conditions, and turn on the pump. Allow the system to

equilibrate under these conditions.

Tabl e 3 Choice of Priming Solvents for Different Purposes

Activity Solvent Comments

After an installation Isopropanol Best solvent to flush air out of the

system

After an installation (second

choice)

Ethanol or Methanol Alternative to Isopropanol if no

Isopropanol is available

When switching between reverse

phase and normal phase (both

times)

Isopropanol Best solvent to flush air out of the

system

To clean the system when using

buffers

Bidistilled water Best solvent to re-dissolve salts

After a solvent change Bidistilled water Best solvent to re-dissolve salts

After the installation of normal

phase seals

(P/N 0905-1420)

Hexane +

5% Isopropanol

Good wetting properties

To clean the capillaries Acetone Best solvent to remove impurities

from the capillaries

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Agilent Technologies 1100 Series Manual de Instruções Página 35