Agilent Technologies 1100 Series Manual de Instruções descarregar pdf (Página 41)

1100 Series Capillary LC System Manual 31

Optimizing Performance 2

• To achieve the best flow stability, especially in the micro mode, %Ripple must

be within acceptable values, typically no worse than 2%.

Fused Silica Capillary issues

• When you connect a capillary (especially at the column) press it smoothly

into the fitting to avoid air gaps. Incorrect setting will result in dispersion

causing tailing or footing peaks.

• Be careful when you bend a Fused Silica Capillary. The diameter must not

be smaller than 40 mm.

• When you replace a part, especially a capillary, clean it with Acetone.

• If a fused silica capillary leaks, do not retighten under flow. Set column

flow to zero, reinsert the capillary, tighten and set new column flow.

• Avoid the use of alkaline solutions (pH > 8.5) which can attack the fused

silica from the capillaries.

• Be careful not to crush capillaries when applying module doors.

• A broken capillary can release silica particles into the system (e.g. cell)

causing problems in the system down-stream of the break.

• A blocked capillary can often be unblocked by back-flushing the capillary.

Acetone is a recommended solvent for capillary back-flushing.

NOTE

Do not overtighten the fused silica capillaries. see Chapter 3, “Capillaries and Fittings,”

starting on page 45 for information on installing and handling capillaries.

40 mm

1 2 ... 36 37 38 39 40 41 42 43 44 45 46 ... 195 196

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Agilent Technologies 1100 Series Manual de Instruções Página 41