Agilent Technologies 1100 Series Manual do Utilizador descarregar pdf (Página 47)

Setting up an Analysis

Preparing the HPLC System

10 Click on the pump and VW detector icon (Figure 24 on page 46) to open the

Setup of those modules. Enter the parameters mentioned under “Conditions”

on page 43.

Figure 25 Setting Parameters

11 Pump the water/acetonitrile (30/70 %) mobile phase through the column for

10 minutes for equilibration.

Enter:

Flow - 1.50 ml/min

Stop Time - 6 minutes

Solvent Description

Enter:

Wavelength - 254 nm

Peak width - 2 s

Stop Time - same as pump

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Agilent Technologies 1100 Series Manual do Utilizador Página 47