Agilent Technologies 1100 Series Manual do Utilizador descarregar pdf (Página 46)

Setting up an Analysis

Preparing the HPLC System

6 Purge the pump. For more information see “Priming and Purging the System”

on page 41.

7 Allow the detector at least 15 minutes to provide a stable baseline.

8 For the isocratic pump, fill the solvent bottle with the mixture of HPLC-grade

bidistilled water (30 %) and acetonitrile (70 %).

9 Click on the Load Method button and select

'()B/&0and press OK. The

default LC method parameters are transferred into the 1100 modules.

Figure 24 Loading Default LC Method

refer to step 10 “Setting

Parameters” on page 47

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Agilent Technologies 1100 Series Manual do Utilizador Página 46