Agilent Technologies DP235 Guia do Utilizador descarregar pdf (Página 98)

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Contents ▲ 98 ▼ Index

NOTE

Peak ratios for the RNA ladder may vary from one batch of RNA 6000 ladder to the

next. Assay performance will not be affected by this variation.

5. For the Eukaryote or Prokaryote Total RNA assay, the RNA fragments (either 18S and

28S for eukaryotic RNA or 16S and 23S for prokaryotic RNA) are detected. After

detection, the ratio of the fragment areas is calculated and displayed.

6. To calculate the concentration of the RNA, the area under the entire RNA

electropherogram is determined. The ladder, which provides the concentration/area

ratio, is applied to transform the area values into concentration values.

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