Agilent Technologies DP235 Guia do Utilizador Página 69

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Contents 69 Index
Protein Assays:
Store Protein sample buffer at -20 °C upon arrival. Keep the vial in use at 4 °C to avoid
freeze-thaw cycles.
Allow the dye concentrate to equilibrate to room temperature for 20 minutes before
use, to make sure the DMSO is completely thawed. Protect the dye from light during
that time. Vortex before use.
Allow all other reagents to equilibrate to room temperature for 10 minutes before use.
Use 0.5 ml tubes to denature samples. Using larger tubes may lead to poor results,
caused by evaporation.
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