Agilent Technologies DP235 Guia do Utilizador descarregar pdf (Página 123)

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Contents ▲ 123 ▼ Index

NOTE

With DNA and protein assays, the height of marker peaks is assay dependent. Ladder

peaks are analyzed to calculate a marker peak threshold that is used to locate the

marker peaks in the sample wells. If the marker peaks found using this calculated

method fail to align with those of a sample, the 2100 expert software will use the

minimum peak height threshold setting instead (if this value is lower than the value for

the marker peak). For example, the calculated threshold might be too high to find the

sample's markers if they happen to be very small for some reason. Either no markers

will be found or the wrong peaks will be assumed to be markers and these may not

align with the ladder markers. Consequently, the software attempts to use the

minimum peak height threshold that, if it is set low enough, will catch the real

markers, allowing the sample to align.

NOTE

After alignment, peaks are shown with relative migration times that are different from

the real times with data unaligned.

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