Agilent Technologies DP235 Guia do Utilizador descarregar pdf (Página 104)

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Contents ▲ 104 ▼ Index

7. The standard curve, in conjunction with the markers, is used to calculate protein sizes

for each sample from the migration times measured.

8. To calculate the concentration of the individual proteins in all sample wells, the upper

marker, in conjunction with an assay-specific concentration calibration curve, is applied

to the individual sample peaks in all sample wells.

NOTE

The software allows you to define upper and lower markers yourself. A change in the

selection of the markers will lead to quantitative changes in the calibration procedure,

however, and therefore in the entire data evaluation.

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