Agilent Technologies DP235 Guia do Utilizador descarregar pdf (Página 231)

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Contents ▲ 231 ▼ Index

Histogram Evaluation

The blue histogram is used for gating. High fluorescence in the blue histogram means

that the cells are living (if a life-indicating dye is used). Low fluorescence means the cells

are dead. If you use a nucleic acid dye, you cannot distinguish between living and dead

cells, you can only count all measured cells. See the following image as an example:

The values are displayed in the result table below the blue histogram:

Low fluorescence

value indicates dead

or non-healthy cells

High fluorescence

value indicates living

cells

All measured events

All events in relation to

the blue marker (here

calcein)

Living cells related to all measured cells (high calcein fluorescence)

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