Agilent Technologies DP235 Guia do Utilizador descarregar pdf (Página 440)

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Contents ▲ 440 ▼ Index

When a new chip run starts, the gel view is blank and the first lane—the ladder well—is

selected. As data is acquired, the selection rectangle around the lane will step from left

to right, highlighting the lane that is currently acquiring data.

However, if you select a lane that is earlier in sequence than the current lane, the

highlight will no longer change as new samples are measured but will remain on the

selected lane.

Moving the mouse pointer over a gel-like image will cause sample data to appear next to

a crosshair pointer. What is displayed depends on the type of assay selected:

• With a DNA assay, you will see the base-pair measurements for the area of the lane

beneath the crosshair of the pointer. When the cursor is positioned over a recognized

band, the cursor will change its shape to a target, and the size, concentration, and

molarity are shown in a tool tip window.

• With RNA assays, the size estimate in terms of # of nucleotides (nt) is shown; over

recognized bands, the area and percent of total area is shown.

• With a protein assay, the tool tip window shows the size of the protein (in kDa) and the

relative concentration (in µg/ml).

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