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34 Agilent 1100 Series Valves

4 Valve Applications

Alternating Column Regeneration

Alternating column regeneration is a convenient way to increase the sample

throughput. The Agilent 1100 Series 2 position/ 10 port valve (G1157A) can be

used in combination with the Agilent LC and LC/MS modules to increase the

efficiency in laboratories running large amounts of samples.

Gradient elution is a common technique for separation of complex samples in

liquid chromatography, which requires column regeneration before the

subsequent run is started. Using alternating column regeneration valuable

time for the analysis is saved. Core of the alternating column regeneration is

the 1100 Series 2 position / 10 port valve, which allows simultaneous analysis

on one column while a second identical column is flushed and equilibrated.

The schematic setup is displayed in Figure 19.

Figure 19 Alternating Column Regeneration (schematic setup)

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Agilent Technologies 1100 Series Guia do Utilizador Página 40