Agilent Technologies E5500 Series Guia do Utilizador descarregar pdf (Página 57)

Fragment Library Preparation Using the AB Library Builder

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System: 5500 Series SOLiD

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Systems User Guide

AppendixB Supplemental Procedures

Quantitate the DNA with the NanoDrop

ND-1000 Spectrophotometer

Lift the sampling arm and load 2 µL of Nuclease-free Water onto the lower

measurement pedestal and lower the sampling arm:

5. In the dialog box, click OK and allow the instrument to initialize.

6. Lift the sampling arm and use Kimwipes

to remove water from the

measurement pedestal and the sampling arm.

7. Load 2 µL of the same buffer that was used to resuspend or elute the DNA onto

the measurement pedestal and lower the sampling arm.

8. Click Blank and allow the instrument to take a measurement:

9. Lift the sampling arm and wipe away the buffer from both the upper and lower

measurement pedestals with Kimwipes

. The instrument is now ready to take

readings.

10. Load 2 µL of DNA sample onto the lower measurement pedestal and lower the

sampling arm.

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Agilent Technologies E5500 Series Guia do Utilizador Página 57