Agilent Technologies E5500 Series Guia do Utilizador Página 1

USER GUIDEFragment Library Preparation Using theAB Library Builder™ System5500 Series SOLiD™ SystemsPublication Part Number 4460965 Rev. ARevision Dat

10Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter1 About the ProductsProduct inform

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12Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter1 About the ProductsKit contents a

13Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER2 Prepare to Build the LibraryWor

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16Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibraryShea

17Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

18Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

19Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.This user guide is the proprietary material of Applied Biosy

20Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

21Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

22Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

23Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

24Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

25Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

26Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter2 Prepare to Build the LibrarySet

27Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER3 Build the libraryWorkflowFor ad

28Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter3 Build the librarySet up for a ne

29Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER4 Nick-Translate the Library with

Contents3Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide About This Guide . . . . . . . .

30Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with

31Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with

32Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with

33Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with

34Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter4 Nick-Translate the Library with

35Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER5 TroubleshootingFor symptoms oth

36Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter5 TroubleshootingRun stops after a

37Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter5 Troubleshooting After the automa

38Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideChapter5 TroubleshootingInstrument error

39Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAAPPENDIXA Ordering InformationThis appe

4Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideContentsAPPENDIX A Ordering Information .

40Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired Ap

41Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired Ap

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43Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired eq

44Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationOptional eq

45Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationRequired co

46Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixA Ordering InformationOptional co

47Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideBAPPENDIXB Supplemental ProceduresThis a

48Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresAB Libra

49Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresAB Libra

5Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideContents Glossary . . . . . . . . . . . . .

50Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresAB Libra

51Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental Procedures(Optiona

52Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental Procedures(Optiona

53Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental Procedures(Optiona

54Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental Procedures(Optiona

55Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresQuantita

56Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresQuantita

57Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresQuantita

58Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixB Supplemental ProceduresQuantita

59Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCAPPENDIXC Supplemental BackgroundInform

6Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideContents

60Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixC Supplemental Background Informa

61Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixC Supplemental Background Informa

62Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixC Supplemental Background Informa

63Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideDAPPENDIXD Library ConstructionOligonucl

64Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

65Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

66Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

67Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

68Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

69Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

7Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide About This GuideSafety informationNote: For

70Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

71Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

72Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixD Library Construction Oligonucle

73Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideEAPPENDIXE Checklist and workflow tracki

74Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixE Checklist and workflow tracking

75Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixE Checklist and workflow tracking

76Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixE Checklist and workflow tracking

77Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideFAPPENDIXF SafetyThis appendix covers:

78Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixF SafetySDSsFChemical safety guid

79Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixF SafetyChemical waste safetyF WA

8Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAbout This GuideSafety informationSDSs The S

80Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideAppendixF SafetyBiological hazard safetyF

81Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide Documentation and SupportRelated documenta

82Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideDocumentation and SupportObtaining support

83Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide Glossarybarcode A short, unique sequence t

84Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideGlossaryP1-T AdaptorA T-tailed double-stran

85Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User Guide IndexAAB Library Builder Systemrunning 27A

86Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideIndexNNanoDrop® ND-1000 Spectrophotometer 5

4460965AHeadquarters5791 Van Allen Way | Carlsbad, CA 92008 USA | Phone +1 760 603 7200 | Toll Free in USA 800 955 6288For support visit www.app

9Fragment Library Preparation Using the AB Library Builder™ System: 5500 Series SOLiD™ Systems User GuideCHAPTER1 About the ProductsNote: For comp