Agilent Technologies G1978B Guia do Utilizador

Agilent TechnologiesAgilent G1978B Multimode Source for 6100 Series Single Quad LC/MSUser Guide

10 Multimode Source for 6100 Series Single Quad LC/MS User Guide1 Basic Operation and Maintenance To create a method for positive/negative mixed mode

Multimode Source for 6100 Series Single Quad LC/MS User Guide 11Basic Operation and Maintenance 1 To create a method for alternating ESI and APCI oper

12 Multimode Source for 6100 Series Single Quad LC/MS User Guide1 Basic Operation and Maintenance To create a method for alternating ESI and APCI ope

Multimode Source for 6100 Series Single Quad LC/MS User Guide 13Basic Operation and Maintenance 1 MaintenanceMaintenanceTo open the multimode sourceOp

14 Multimode Source for 6100 Series Single Quad LC/MS User Guide1 Basic Operation and Maintenance To open the multimode source7 In the LC/MS ChemStat

Multimode Source for 6100 Series Single Quad LC/MS User Guide 15Basic Operation and Maintenance 1 To turn off the instrumentTo turn off the instrument

16 Multimode Source for 6100 Series Single Quad LC/MS User Guide1 Basic Operation and Maintenance To clean the multimode source dailyTo clean the mul

Multimode Source for 6100 Series Single Quad LC/MS User Guide 17Basic Operation and Maintenance 1 To clean the multimode source daily5 Wipe the interi

18 Multimode Source for 6100 Series Single Quad LC/MS User Guide1 Basic Operation and Maintenance To clean the multimode source daily11 Close the spr

Multimode Source for 6100 Series Single Quad LC/MS User Guide 19Basic Operation and Maintenance 1 To clean the multimode source weeklyTo clean the mul

2 Multimode Source for 6100 Series Single Quad LC/MS User GuideNotices© Agilent Technologies, Inc. 2006No part of this manual may be reproduced in any

20 Multimode Source for 6100 Series Single Quad LC/MS User Guide1 Basic Operation and Maintenance To clean the multimode source weekly6 Remove the sp

Multimode Source for 6100 Series Single Quad LC/MS User Guide 21Basic Operation and Maintenance 1 To bake out the LC/MSTo bake out the LC/MS You will

22 Multimode Source for 6100 Series Single Quad LC/MS User Guide1 Basic Operation and Maintenance To do an autotuneTo do an autotuneTuning the multim

23Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MSUser GuideAgilent Technologies2InstallationStep 1. Prepare to install 24Step 2.

24 Multimode Source for 6100 Series Single Quad LC/MS User Guide2 Installation Step 1. Prepare to installStep 1. Prepare to installThe Multimode Enab

Multimode Source for 6100 Series Single Quad LC/MS User Guide 25Installation 2 Step 2. Install the HV control PCA and cablesStep 2. Install the HV con

26 Multimode Source for 6100 Series Single Quad LC/MS User Guide2 Installation Step 2. Install the HV control PCA and cables6 Install the APCI HV pow

Multimode Source for 6100 Series Single Quad LC/MS User Guide 27Installation 2 Step 2. Install the HV control PCA and cables Figure 13 Conn

28 Multimode Source for 6100 Series Single Quad LC/MS User Guide2 Installation Step 2. Install the HV control PCA and cables Figure 16 Installing t

Multimode Source for 6100 Series Single Quad LC/MS User Guide 29Installation 2 To remove the multimode sourceTo remove the multimode sourceDo the foll

Multimode Source for 6100 Series Single Quad LC/MS User Guide 3In This GuideThis guide explains how to install, maintain and troubleshoot your nanoele

30 Multimode Source for 6100 Series Single Quad LC/MS User Guide2 Installation To convert from multimode to ESI, APCI or APPITo convert from multimod

Multimode Source for 6100 Series Single Quad LC/MS User Guide 31Installation 2 To convert from ESI, APCI or APPI to the multimode sourceTo convert fro

32 Multimode Source for 6100 Series Single Quad LC/MS User Guide2 Installation To convert from ESI, APCI or APPI to the multimode sourceFigure 17 Sta

Multimode Source for 6100 Series Single Quad LC/MS User Guide 33Installation 2 To convert from ESI, APCI or APPI to the multimode sourceFigure 19 Mult

34 Multimode Source for 6100 Series Single Quad LC/MS User Guide2 Installation To convert from ESI, APCI or APPI to the multimode sourceFigure 21 Mul

Multimode Source for 6100 Series Single Quad LC/MS User Guide 35Installation 2 To convert from ESI, APCI or APPI to the multimode sourceFigure 23 No n

36 Multimode Source for 6100 Series Single Quad LC/MS User Guide2 Installation To convert from ESI, APCI or APPI to the multimode source18 Connect th

37Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MSUser GuideAgilent Technologies3ReferenceSafety and Specifications 38 Safety

38 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Safety and SpecificationsSafety and SpecificationsThis section describes

Multimode Source for 6100 Series Single Quad LC/MS User Guide 39Reference 3 Safety• The Infrared medium-wave twin tube emitters are made of quartz gla

4 Multimode Source for 6100 Series Single Quad LC/MS User Guide

40 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Safety• Fluid drained from the multimode chamber is made of solvent and s

Multimode Source for 6100 Series Single Quad LC/MS User Guide 41Reference 3 SafetyCleanlinessCleanliness and the prevention of accidental contaminatio

42 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Safety SymbolsSafety SymbolsNOTEThis symbol is placed on the product wher

Multimode Source for 6100 Series Single Quad LC/MS User Guide 43Reference 3 Technical SpecificationsTechnical SpecificationsSizeWeight 2.27 kg / 5 lbs

44 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference OverviewOverviewBenefits of a multimode sourceFigure 26 Multimode source.

Multimode Source for 6100 Series Single Quad LC/MS User Guide 45Reference 3 Benefits of a multimode source• Permits analysis of fast-eluting peaks• Op

46 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Benefits of a multimode sourceMultimode source operational descriptionFig

Multimode Source for 6100 Series Single Quad LC/MS User Guide 47Reference 3 Benefits of a multimode sourceMM-ES ZoneFigure 28 Graphical representation

48 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Benefits of a multimode sourceInfrared DryingFigure 29 Graphical represen

Multimode Source for 6100 Series Single Quad LC/MS User Guide 49Reference 3 Benefits of a multimode sourceMM-APCI ZoneFigure 30 Graphical representati

Contents Multimode Source for 6100 Series Single Quad LC/MS User Guide 5Content1 Basic Operation and Maintenance 7Setting up Methods 8To set

50 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Benefits of a multimode sourceMultimode source side viewFigure 31 Graphic

Multimode Source for 6100 Series Single Quad LC/MS User Guide 51Reference 3 Benefits of a multimode sourceMultimode source top down viewFigure 32 Grap

52 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Mobile phase considerations when using the multimode sourceMobile phase c

Multimode Source for 6100 Series Single Quad LC/MS User Guide 53Reference 3 Mobile phase considerations when using the multimode source• In negative A

54 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Flowchart summarizing parameter choices for different modesFlowchart summ

Multimode Source for 6100 Series Single Quad LC/MS User Guide 55Reference 3 Source comparison in positive ion modeSource comparison in positive ion mo

56 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Source comparison in negative ion modeSource comparison in negative ion m

Multimode Source for 6100 Series Single Quad LC/MS User Guide 57Reference 3 Single analysis in MM-ES+APCI mode with fast switchingSingle analysis in M

58 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference GuidelinesGuidelinesGuidelines for multimode source settings in MM-ES mod

Multimode Source for 6100 Series Single Quad LC/MS User Guide 59Reference 3 Guidelines for multimode source settings in MM-APCI modeGuidelines for mul

6 Multimode Source for 6100 Series Single Quad LC/MS User GuideContents Source comparison in negative ion mode 56Single analysis in MM-ES+APCI mo

60 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Guidelines for multimode source settings in MM-ES+APCI modeGuidelines for

Multimode Source for 6100 Series Single Quad LC/MS User Guide 61Reference 3 Guidelines for positive/negative polarity switching in mixed modeGuideline

62 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Guidelines for positive/negative polarity switching in mixed modeNOTEThe

Multimode Source for 6100 Series Single Quad LC/MS User Guide 63Reference 3 Empirical Formulas for high resolution MSEmpirical Formulas for high resol

64 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference G2422A MM-APCI Calibration IonsG2422A MM-APCI Calibration Ions NOTEFragme

Multimode Source for 6100 Series Single Quad LC/MS User Guide 65Reference 3 Tuning Mix and Test MixTuning Mix and Test MixG2421A ES Tuning Mix (100mL)

66 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference G2422A APCI Tuning Mix (100mL)G2422A APCI Tuning Mix (100mL)Item-Descript

Multimode Source for 6100 Series Single Quad LC/MS User Guide 67Reference 3 Chemical structures in the multimode test mixChemical structures in the mu

68 Multimode Source for 6100 Series Single Quad LC/MS User Guide3 Reference Chemical structures in the multimode test mix

Multimode Source for 6100 Series Single Quad LC/MS User Guide 69IndexIndexAAPCI Tuning Mix, 66autotune, 22BBake out the LC/MSD, 21Cchemical structu

7Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MSUser GuideAgilent Technologies1Basic Operation and MaintenanceSetting up Methods

70 Multimode Source for 6100 Series Single Quad LC/MS User GuideIndex

© Agilent Technologies, Inc. 2006Printed in USA First Edition, September 2006*G1978-90020*G1978-90020www.agilent.comAgilent TechnologiesIn This BookT

8 Multimode Source for 6100 Series Single Quad LC/MS User Guide1 Basic Operation and Maintenance Setting up MethodsSetting up MethodsThis section des

Multimode Source for 6100 Series Single Quad LC/MS User Guide 9Basic Operation and Maintenance 1 To create a method for positive/negative mixed mode o