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Lab Exercise: Running a Sequence
Building a Sequence
102
7) Now enter the Sequence Table from the Sequence menu or from the
sequence tray setup tool. You would like to do the following:
a) Replace all existing calibration table response factors with new response
factors at all three calibration standard levels.
b) There after, perform cyclic recalibrations every 2 sample injections
averaging the response factors and retention times.
c) Perform four sample injections, in this case utilize the vial containing your
isocratic test mix and make four injections from this vial.
Fill in the table. Each time you wish to add a new line to the bottom of the table,
highlight the last line by clicking on the line number, then use the Append Line
button. To add a line above the currently highlighted line, use the Insert button.
The table should look like the one below. You can also try to use the
Insert/FillDown Wizard.
- Agilent 1100/1200 HPLC 1
- ChemStation Operation 1
- Course Number H4033A 3
- Laboratory Manual 3
- Table Of Contents 5
- ChemStation 9
- In this Laboratory You Will: 10
- Configuration Editor 13
- Scheduler 14
- Windows XP) 16
- Cleaning up Temporary Files 16
- Repairing the Disk 16
- Disk Defragmentation 17
- Workstation) 18
- (Windows 2000 or XP) 21
- Windows Explorer 21
- Wallpaper and Screen Savers 22
- Clipboard 23
- Preparation 27
- Preparing the HPLC 28
- Practice Entering Gradients 32
- OK the dialog box 34
- Snapshot 35
- Graphics - Signal Options 39
- Graphics - Annotation 41
- Window Functions 43
- Peak Purity 44
- Displaying Spectra 47
- Isoabsorbance Plot 48
- 3D Plot 50
- Building a Library 53
- Library Searching 55
- The Agilent 1200 Flow Path 59
- Diagnosis View 61
- Diagnosis Instrument Panel 62
- Instrument Tests 64
- Routine Maintenance 67
- Agilent LC Diagnostic Tool 73
- Diagnosing Common Problems 74
- Instrument Status Report 77
- Lab Exercise: Integration 81
- Integration Preparation 83
- Auto Integration 84
- Adding Timed Events 87
- Using Manual Events 89
- Data Acquisition-Standards 97
- Integration 99
- Setting up Signal Details 101
- Building a Calibration Table 102
- Calibration Standards 104
- Building a Sequence 109
- Sequence Summary Reports 112
- Starting the Sequence 113
- Pausing a Sequence 114
- Post Sequence 115
- Reprocessing 117
- Reprocess with DA Methods 125
- Batch Review 129
- Adding a Report Header 133
- Automated Peak Purity 137
- Preparations 143
- Building a Customized Report 144
- Adding a General Section 145
- Inserting Tables 146
- Adding a Calibration Curve 147
- Finishing Up 148
- Lab Exercise: Commands 149
- In this LaboratoryYou Will: 150
- Commands 152
- Registers 153
- Objects 154
- Lab Exercise: Macro Writing 161
- Write Your Own Macro 165
- Possible Answer 166
- Agilent ChemStation 168
- Getting Started with New 168
- ChemStation Workflow 168
- In This Guide… 170
- 4 Data Organization 171
- Contents 172
- 6 Data Organization 173
- ChemStation Data Structure 174
- ChemStation prior to B.02.01 175
- ChemStation B.02.01/B.03.01 176
- 12 Data Organization 179
- 1 ChemStation Data Structure 179
- Data Acquisition 180
- 18 Data Organization 185
- 2 Data Acquisition 185
- Data Analysis 186
- 22 Data Organization 189
- 3 Data Analysis 189
- B.02.01 and higher 189
- Data Analysis: Data Review 190
- Keep “Current Method” 191
- Load “Sequence Method” 192
- Update master method feature 196
- Update Sequence Method 197
- Master method — 197
- Data Organization 33 200
- Data Analysis 3 200
- 34 Data Organization 201
- Agilent Technologies 202
- 42 Data Organization 209
- *G2170-90041* 211
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