Agilent Technologies 6612B Guia do Utilizador

Agilent EZChrom Elite User’s Guide

10 User’s Guide Create a sequence This button starts the Sequence Wizard that steps you through creation of an acquisition or reprocessing sequence. R

100 User’s Guide 6. Leave the Amount Values set to "1". For details on how these values are used, see Method Development. 7. Click on the

User’s Guide 101 2. Type the method to be used for the acquisition, or select the name from a list of available methods by clicking the File Open b

102 User’s Guide 5. The Sample ID field is for an identification for the samples. Click the blue right arrow and select the Line number and Method na

User’s Guide 103 10. In this dialog, the calibration ID and calibration file names are automatically set to the identifications from the previous d

104 User’s Guide 11. Select the check boxes to cause the samples to be included in a summary report and calibration summary report. Do not select th

User’s Guide 105 Sample ID’s and Data File names are numbered automatically to prevent duplication. In order to run a calibration standard as the fir

106 User’s Guide Enter the name of your sequence file by typing the name, along with path, in the Sequence Name field. You can also select it from a

User’s Guide 107 level calibration, use the multilevel calibration.met file provided with the data system. 1. Open the multilevel calibration.met me

108 User’s Guide for the different fit types displayed are shown, along with the goodness of fit calculation, r2, which is not calculated for the Poin

User’s Guide 109 including the Levels, which contain the calibration amounts for each compound at each level of calibration. Note that it is possible

User’s Guide 11 4 Client/Server Operation When operating in a Client/Server mode, you will have one or more Client Workstations along with one or m

110 User’s Guide If you wish, you can modify the standard report templates, or create entirely new reports using the Custom Report feature. You can cr

User’s Guide 111 Changing Integration Parameters Another important aspect of using a computerized data system is the ability to customize the integra

112 User’s Guide 3. Add the Valley-to-Valley timed event to integrate the cluster of 4 large peaks with valley-to-valley baselines. To do this, click

User’s Guide 113 4. Click the Integration Events Table button from the command ribbon. Note the addition of the Valley to Valley event in the table.

*G4650-90008 G4650-90008

12 User’s Guide

User’s Guide 13 To turn the Navigation pane on 1. Select View followed by the Navigation pane command. To turn the Navigation pane off 1. Click t

14 User’s Guide Views Data Display options, Manual Integration Fixes, Baseline Check Status 7 Data Acquisition Using Agilent Instrument Controllers

User’s Guide 15 workstation (if the user has appropriate privilege assignment). In order for sequence changes to take effect, the sequence must be sa

16 User’s Guide About Data File Structure A data file is created on the designated drive whenever you acquire a sample, or when you save a data file u

User’s Guide 17 first. If the check fails, the file cannot be opened and an error message will appear in the instrument activity log. Checksum verifi

18 User’s Guide and cannot be removed or overwritten. To view the changes logged in the Audit Trail for a method, 1. From the File menu, select Meth

User’s Guide 19 9 Opening and Saving Files Open Data Files Whenever you open a file using the data system, you will be presented with a dialog box

Notices Copyright © Scientific Software, Inc 1997-2005 © Agilent Technologies, Inc. 2006-2008. No part of this manual may be reproduced in any form or

20 User’s Guide systems with advanced file security enabled. Users will be limited to storing files within the current project folder and the Enterpri

User’s Guide 21 Results When one of the Results options is selected, the data file will be opened along with the selected results. When a data file i

22 User’s Guide Note: When using the Search feature, make sure the Windows Hide File Extensions for Known File Types option is turned OFF. To turn

User’s Guide 23 Searching for Method and Sequence Files The criteria you can use to search for specific method and sequence files include selection o

24 User’s Guide To save the current data in a new data file, type the name of the new data file in the File name field, then click Save. Use the butt

User’s Guide 25 10 Reading CDF Files When opening a CDF file, the software looks for one of the following Y-axis labels: "microvolts", &qu

26 User’s Guide Enter the information as prompted, and then click the Login button. User name: Enter the user name which has been assigned to you on

User’s Guide 27 If you select this box, the password you type at login will be saved when you click the Login button. Once the password has been save

28 User’s Guide To view details of any line in the instrument activity log, click on the line to highlight it, then do a right mouse click within the

User’s Guide 29 • Instrument control and data acquisition • Analysis and review of data • Reporting • Data export You can customize the appearanc

User’s Guide iii Contents 1 Using This Guide ... 8 Introduction ...

30 User’s Guide Preferences - General This tab is used to set up general preferences in the instrument window. Toolbar options For each area of the w

User’s Guide 31 2. Click Files to change the file view preferences for the instrument window. 3. Select the file type, then enter the number of fil

32 User’s Guide 12 The Chromatogram Window About The Chromatogram Window Whenever there is data to be viewed, it is shown in a chromatogram window.

User’s Guide 33 As data is being acquired, it is also displayed in a chromatogram window. At the end of a run the data becomes the "current da

34 User’s Guide Zooming You may want to examine a chromatogram in more detail, or zoom in on a portion of the chromatogram. To do this, drag a box aro

User’s Guide 35 Add a Trace (Viewing Multiple Chromatograms) The chromatogram window is used to view data, either current data (real-time) in the ins

36 User’s Guide 3. Select the New Trace tab. Fill in the fields to add a trace to the chromatogram window and set its properties. These properties a

User’s Guide 37 Current Data This selection allows you to select a trace from the current chromatography data. Current Method This selection enables

38 User’s Guide Y min If you have selected a User Defined scale, enter a minimum value for the Y-axis. Y max If you have selected a User Defined scale

User’s Guide 39 3. Once you have added a data file to the list, you can select the channel by clicking on the Trace field, then click the down-arro

iv User’s Guide Open Data File Options... 20 Open Method and Sequence Files

40 User’s Guide Annotate a Chromatogram To change the annotations on the chromatogram, 1. From the chromatogram window, do a right mouse click and t

User’s Guide 41 3. Select the trace from the drop-down list. Then select what features you wish to annotate. Normal choice is Peaks. However, if yo

42 User’s Guide 7. Click the check box(s) to display Baseline, USP Width, or Retention Time Windows, Show undetected named peaks, and Group ranges on

User’s Guide 43 Scheme If you have previously saved an appearance scheme on disk, you can select it from this box. The Save As… button allows you to

44 User’s Guide Graph, you will have access to setting up appearances of sub-items including the background, axes and labels for the graph. If the ite

User’s Guide 45 Sub-items available in the Appearance tab are as follows. Item Sub-Item Description Graph Background Select the color of the grap

46 User’s Guide Graph Legend Select a color and/or font for display of the graph legend. The legend indicates what traces are currently displayed in

User’s Guide 47 Change the Axis Properties The Axis Setup tab allows you to configure the appearance of the axis on your chromatogram. These settings

48 User’s Guide Axis Using the drop-down list, select the axis of interest: Left Y-Axis, Right Y-Axis, or X-Axis. Then for your selection, you can cho

User’s Guide 49 Chromatogram Window with Legend and Grid displayed Chromatogram Window with no Legend or Grid displayed Orientation Select portrai

User’s Guide v Set up a trace...50 Remove a Trace

50 User’s Guide in the Data Graph Properties. To change the Data Graph Properties, 1. In the Chromatogram Window, do a right mouse click and select

User’s Guide 51 Show Click this box to show the trace in the chromatogram window. De-select this box to remove the trace from the display (but leavi

52 User’s Guide the legend (color, etc.) is done in the Appearance tab for the Graph item. Note: If you have not turned on the Legend in the Axis Setu

User’s Guide 53 Autoscale to 3rd largest peak Scales such that the 3rd largest peak is on scale. User Defined Allows you to enter a value for Y m

54 User’s Guide Hide Details Click this button to hide the current trace details and display only the spreadsheet. Reset Scaling Click this button to

User’s Guide 55 3. Click the Axis Setup tab to set absolute ranges for the trace. Select X-Axis, to set the range for the X-Axis. Click Autoscale t

56 User’s Guide always be displayed in the chromatogram window using these ranges until you change or reset them. 5. To reset the scaling of all chr

User’s Guide 57 13 Chromatogram Operations About Chromatogram Operations There are a number of chromatogram comparison and mathematical operations t

58 User’s Guide Operation Action Move Trace Lets you "grab" and move a trace within the chromatogram window. Stack Traces Positions multi

User’s Guide 59 Move a Trace To "grab" a trace and move it with your mouse 1. In the chromatogram window, do a right mouse click and selec

vi User’s Guide 15 Data Acquisition and Control... 74 Data Acquisition and Control...

60 User’s Guide Chromatograms before stacking Chromatograms after stacking To remove these offsets, 1. In the chromatogram window, click the right

User’s Guide 61 Align two traces To Align one chromatogram to another, 1. In the chromatogram window, do a right mouse click, and then select Opera

62 User’s Guide First peak of top chromatogram aligned to first peak on bottom chromatogram. Stretch a chromatogram The stretch function allows you t

User’s Guide 63 Chromatograms before stretching. Bottom chromatogram stretched relative to top chromatogram. Normalize Traces This function allows

64 User’s Guide To un-do the normalization, use the right-hand mouse button/ Properties command to view the trace spreadsheet. Click the Trace Setup t

User’s Guide 65 Perform Mathematical Operations on Traces Performing mathematical operations on traces can be done from within the chromatogram windo

66 User’s Guide Smoothed result trace is displayed with original trace. Calculate Derivatives To calculate and display the 1st or 2nd derivative of a

User’s Guide 67 Trace before 1st derivative. 1st derivative trace displayed with original trace.

68 User’s Guide 2nd Derivative displayed with original trace Add two traces To add two traces to a chromatogram window, 1. In the chromatogram wind

User’s Guide 69 Note: In order for this operation to be valid, both traces must have the same sampling frequency. Multiply Traces To multiply two t

User’s Guide vii Examine a Custom Report ...109 Changing Integration Para

70 User’s Guide ymult = the y multiplier for the trace that converts it from microvolts to the trace's displayed units Utilities About Chromatog

User’s Guide 71 Print a trace This command sends the current chromatogram window view to the printer. Copy to clipboard To copy the contents of the w

72 User’s Guide Parameters that can be set using graphical programming include: Command Action Width Inserts a Width event at the point on the chroma

User’s Guide 73 Force Peak Stop Force the end of a peak. Move Baseline Manually move a baseline. Reset Baseline Force a baseline to the point. Re

74 User’s Guide 15 Data Acquisition and Control Data Acquisition and Control Commands that are available from the Control menu are related to data ac

User’s Guide 75 Run Information This section allows you to specify files for the run. Sample ID Enter a Sample ID for the run. This can contain text

76 User’s Guide field. It is not possible to use an existing file name, unless the file exists in located in a directory whose path contains the term

User’s Guide 77 Internal Standard Amount For calibration runs, the Internal Standard Amount is taken from the method Peak Table. For unknown runs, en

78 User’s Guide Clear replicates Click this box if you want to clear all existing replicates from the existing calibration level before running the sa

User’s Guide 79 Run a Sequence Once you have created and saved a sequence, you can use it to acquire and process data. To start a sequence acquisitio

8 User’s Guide 1 Using This Guide Introduction This User’s Guide provides a basic operating overview and Tutorial for the Agilent EZChrom E

80 User’s Guide Range Enter a range of runs to be executed. For example, an entry of 4 - 6 will execute runs 4, 5, and 6 of the sequence. An entry of

User’s Guide 81 Seq. w/ Back Calc Select this if you want to perform the sequence mode of bracketing calibrations and back-calculate calibration runs

82 User’s Guide Select the On Stop or Error box to send email notification when the sequence stops or if an error occurs. When you have completed the

User’s Guide 83 Stop current run and sequence run This selection stops the run currently in progress, and terminates the sequence it is a part of. Ot

84 User’s Guide Note: If you are not the user that submitted the run or sequence or are using an instrument offline, you do not have access to th

User’s Guide 85 17 Tutorial This section walks you through the basics of using EZChrom Elite. Follow the steps to set up a method and acquire a data

86 User’s Guide Step 4: Using Tutorial Files • Review a Multi-level Calibration • Explore a Peak Table • Examine a Custom Report • Change integra

User’s Guide 87 When you click this button, the Method Wizard will appear, allowing you to select how you want to use the wizard. Select the Create

88 User’s Guide When you select this button, the Method Wizard sets up a bank of buttons in your application window that allow you to "step"

User’s Guide 89 The default Sampling Frequency should be adequate for most types of chromatography. For Frequency data sampling, the data rate is s

User’s Guide 9 2 Basics of Operation This section describes the basic operation of EZChrom Elite, its file structure, features of the application wi

90 User’s Guide None Sampling of data starts immediately when Start is clicked. Manual User must start the data sampling. External Data sampli

User’s Guide 91 Select the folder in which you want to save your file. In the Filename field, type Test.met as the filename for saving the method.

92 User’s Guide 2. At this point, enter Test in the Sample ID field. 3. In the Method field, either type your method name, along with its path, or

User’s Guide 93 into your method as Integration Timed Events. These events can be placed at the beginning of the run to apply to all peaks, or they c

94 User’s Guide The points where you clicked your mouse are shown as Start Time and Stop Time. The integration will be turned off between these point

User’s Guide 95 the row number, then press the Delete key on your keyboard. You can also delete the event using the Edit/Cut command. Using this comm

96 User’s Guide integration the chromatogram and show the baselines. 2. Click the Define Single Peak button on the Toolbar. A dialog box will appear

User’s Guide 97 would enter Concentration Level 2 and the amount for that level. Continue to enter level concentrations until you have completed the

98 User’s Guide the chromatogram is displayed on the right of the dialog box. When you are finished adding peaks to your peak table, click Done. Each

User’s Guide 99 Calibrate Using a Stored Data File In order for the software to calculate amounts for unknown samples, your method must contain the a